Protein Extraction From Plant

              Plant Protein Extraction 

Plant protein extraction this process involves homogenization of the plant leaf sample followed by acetone precipitation for protein extraction.

 Leaf homogenization:                                                                                                                              
  • Select the plant leaf sample of interest and weigh around 300 mg of the leaf on an aluminum foil.
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Plant: Delonix regia
                                                                    
  • Transfer these leaves to a chilled mortar and carefully add liquid nitrogen to it which helps in drying up the leaves instantaneously.
  •  Grind the leaves well using a pestle to obtain a fine powder to this powder add around 0.5 ml of lysis buffer containing trichloroacetic acid acetone and dio3 toll and grind it well until a fine paste is obtained the lysis buffer causes the plant cells to swell and finally break open thereby disrupting the membrane and releasing all its intracellular contents.
  •  Add another 1ml of lysis buffer to the paste and then transfer the solution into a fresh tube. After grinding thoroughly to obtain a uniform mixture.
  • Centrifuge the sample at 10,000 rpm for 5 minutes and maintain a temperature of 4 degrees Celsius during the process to ensure that there is no denaturation of the proteins.


  • Discard the supernatant and retain the pellet containing plant proteins along with various other intracellular components incubate the pellet at -20 degrees Celsius for an hour.
       Acetone precipitation of protein:
  • Protein precipitation remove the pellet from -20 degrees Celsius and add chilled acetone to it mix the sample well by vortexing to obtain a uniform solution centrifuge the contents at  4 degrees Celsius for 5 minutes at a speed of 10,000 rpm.
  • Discard the supernatant and repeat the acetone washing at least 3 times to remove all plant pigments obtain protein pellet at room temperature reconstitute this pellet with rehydration buffer vortex the sample to obtain a uniform solution centrifuge the contents the following morning at 10000 rpm for 5 minutes.


  • Collect the supernatant containing proteins in a fresh tube and store it at -20 degrees Celsius until protein quantification is performed.


                                           Hopeful you may like it!!


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