Post Translational Modification Protein after or during translation undergo several modifications to become functional, which are discussed as under: During and after its synthesis a polypeptide chain begin to coil and fold spontaneously forming a functional protein of specific conformation. A gene determines primary structure, and primary structure in turn determines conformation. In many cases, chaperone proteins help the polypeptide to fold correctly. Additional steps such as post-translational modifications may be required before the protein can begin doing its particular job in the cell. Enzymes may remove one or more amino acids from the leading (amino) end of the polypeptide chain .In some cases, a single polypeptide chain may be enzymatically cleaved into two or more pieces. For examples, the protein insulin is first synthesized as a single polypeptide chain, but becomes active only after an enzyme exercise a central part of the chain leaving a protein made up of ...
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