DIFFERENCE BETWEEN PROKARYOTIC DNA REPLICATION AND EUKARYOTIC DNA.

  • EUKARYOTIC DNA REPLICATION:
  1. Due to the large size of eukaryotes, they posses 25 times more DNA.
  2. Eukaryotic cells have multiple point of origin and they use unidirectional replication within the nucleus of the cell.
  3. Eukaryotic cells have four or more types of polymerase.
  4. Replication of eukaryotic cells is slower taking up to 400 hours.
  5. .Eukaryotes have a distinct process for replicating the telomeres at the ends of their chromosomes.
  6. Eukaryotic cells only undergoes DNA replication during the S-phase of the cell cycle.


                        
  • PROKARYOTES DNA REPLICATION:

  1. Due to its small size, they have very minimal/little DNA.
  2. Prokaryotes cells have a single point of origin and replication takes place in two opposite directions at the  same time and it takes place in the cell cytoplasm.
  3. Prokaryotes cells possess one or two types of polymerases.
  4. Replication in prokaryotes cells is faster, taking up to 40 minutes.
  5. Prokaryotes have circular chromosomal DNA therefore they do not have any ends to synthesize.    
  6. Replication in prokaryotes takes place almost continuously. 

Comments

Post a Comment

Popular posts from this blog

Plant DNA extraction - CTAB Method

Image
  Plant DNA extraction - CTAB Method   An extraction of DNA from plant samples using c tab method 100 ml of setup lysis purple can be prepared using this description after collection of plant samples i have to measure 200 milligram and grind it with liquid nitrogen then transfer all ground plant samples into an append of tube and add 700 microliters of sit up lysis buffer vortex the mixture then incubate at 65 degrees Celsius for 20 minutes in a water bath after incubation centrifuge samples at 10 000 rpm for 10 minutes after centrifuge transfer supernatant to a clean tube and add an equal volume of chloroform and isoamyl alcohol after addition of chloroform and iso-mine alcohol mix it well using vortex and centrifuge at 10 000 rpm for 10 minutes after centrifuge you will see an aqueous upper layer and an organic bottom layer now transfer the aqueous layer to a clean tube while transferring the quest layer...
Read more

Separation of Photosynthetic Pigments by Chromatography

Image
How you can separate leaf pigments by a process known as chromatography?  In this example I'm going to use spinach leaves so the first step is for you to obtain your plant that contains the pigments you wish to separate break up one of the leaves and de little pieces and place it in a mortar add a few drops of acetone and a little bit of sand as this helps with grinding up the leaves use the pestle mortar to grind up the leaves and to release the pigments if it helps here  you can add a few more drops of acetone but ultimately the objective is to obtain a concentrated pigment in a liquid at the bottom of the mortar. Chromatography Paper after you've done this then you need to prepare your chromatography paper now this does actually work best with thin layer chromatography paper but you can also use regular chromatography paper to prepare you're going to obtain a pencil and it is important that it's a pencil not a pen because a pen contains other pigments use the pencil ...
Read more

USING OF MICROCENTRIFUGE

Image
 How To Use Micro centrifuge machine in lab? microcentrifuge         Using of micro centrifuge:      You are going to use a new piece of lab equipment today it is a micro centrifuge . if you push down on here it will open the lid please don't just try to pry it open you have to push this and then you can gently lift the lid and then it has a power button but it won't turn on unless you plug it in there's the power supply on the back you'll be given your power cord just like the heat block plug it in .         So, when you use this it's very important that the tubes are placed in here across from each other to balance so you will be placing one milliliter of your yeast solution to start with again stir it make sure it's good and stirred so the micro pipette you've used this but you've never really measured with it so this mark right here is very small it's the last mark at the top that is one milliliter so you will apply a litt...
Read more