Benedict’s Test for Reducing sugars - Principle, Composition

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Separation of Photosynthetic Pigments by Chromatography

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How you can separate leaf pigments by a process known as chromatography?  In this example I'm going to use spinach leaves so the first step is for you to obtain your plant that contains the pigments you wish to separate break up one of the leaves and de little pieces and place it in a mortar add a few drops of acetone and a little bit of sand as this helps with grinding up the leaves use the pestle mortar to grind up the leaves and to release the pigments if it helps here  you can add a few more drops of acetone but ultimately the objective is to obtain a concentrated pigment in a liquid at the bottom of the mortar. Chromatography Paper after you've done this then you need to prepare your chromatography paper now this does actually work best with thin layer chromatography paper but you can also use regular chromatography paper to prepare you're going to obtain a pencil and it is important that it's a pencil not a pen because a pen contains other pigments use the pencil ...
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Plant DNA extraction - CTAB Method

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  Plant DNA extraction - CTAB Method   An extraction of DNA from plant samples using c tab method 100 ml of setup lysis purple can be prepared using this description after collection of plant samples i have to measure 200 milligram and grind it with liquid nitrogen then transfer all ground plant samples into an append of tube and add 700 microliters of sit up lysis buffer vortex the mixture then incubate at 65 degrees Celsius for 20 minutes in a water bath after incubation centrifuge samples at 10 000 rpm for 10 minutes after centrifuge transfer supernatant to a clean tube and add an equal volume of chloroform and isoamyl alcohol after addition of chloroform and iso-mine alcohol mix it well using vortex and centrifuge at 10 000 rpm for 10 minutes after centrifuge you will see an aqueous upper layer and an organic bottom layer now transfer the aqueous layer to a clean tube while transferring the quest layer...
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Merits and Demerit of BESSEY'S system of classification

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