we'll
discuss an important biochemical test in
the detection of sugars in the urine
which is Benedict's reagent test the aim
is to detect the presence of sugars in a
given sample of urine using the
Benedict's reagent the materials
required will be a Benedict's solution
which serves as a reagent in this testthe reagent is a blend of copper sodium
citrate and sodium carbonate along with
copper to sulfate pentahydrate other
test materials required would be a test
tube a test tube holder a pipette you're
an example and a burner now let's get on
to the procedure of the Benedict's test
using a pipette accurately take 5 ml of
Benedict's reagent and slowly transfer
it to the test tube
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take 5 ml of freshly collected urine by
pit-pat and then added to the test tube
with Benedict's reagent
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the test-tube should be held securely
with the test tube holder to heat it on
the burner for 2 minutes now start
observing the changes on heating the
sample the presence of sugar is
indicated in the sample of urine when a
green precipitate is apparent in the
test tube which denotes traces of
reducing sugars different precipitates
are formed depending upon the sugar
concentration in the urine
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which can be blue
or green
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orange-red
and brick-red each of these colors
denote different types of sugars the
blue precipitate denotes that there is
no sugar in the urine while the green
precipitate shows that there are traces
of reducing sugars the orange and red
denotes that there is moderate amounts
of reducing sugars while brick-red
precipitate denotes that there are large
amounts of reducing sugars that
concludes the Benedict's test for
detecting reducing sugars in the urine. You can also watch video by below link.
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