Benedict’s Test for Reducing sugars - Principle, Composition

we'll

discuss an important biochemical test in

the detection of sugars in the urine

which is Benedict's reagent test the aim

is to detect the presence of sugars in a

given sample of urine using the

Benedict's reagent the materials

required will be a Benedict's solution

which serves as a reagent in this test

the reagent is a blend of copper sodium

citrate and sodium carbonate along with

copper to sulfate pentahydrate other

test materials required would be a test

tube a test tube holder a pipette you're

an example and a burner now let's get on

to the procedure of the Benedict's test

using a pipette accurately take 5 ml of

Benedict's reagent and slowly transfer

it to the test tube

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take 5 ml of freshly collected urine by

pit-pat and then added to the test tube

with Benedict's reagent

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the test-tube should be held securely

with the test tube holder to heat it on

the burner for 2 minutes now start

observing the changes on heating the

sample the presence of sugar is

indicated in the sample of urine when a

green precipitate is apparent in the

test tube which denotes traces of

reducing sugars different precipitates

are formed depending upon the sugar

concentration in the urine

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which can be blue

or green

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orange-red

and brick-red each of these colors

denote different types of sugars the

blue precipitate denotes that there is

no sugar in the urine while the green

precipitate shows that there are traces

of reducing sugars the orange and red

denotes that there is moderate amounts

of reducing sugars while brick-red

precipitate denotes that there are large

amounts of reducing sugars that

concludes the Benedict's test for

detecting reducing sugars in the urine.                                                 You can also watch video by below link.

https://youtu.be/PAKCgrnKeBA