Plant DNA extraction - CTAB Method An extraction of DNA from plant samples using c tab method 100 ml of setup lysis purple can be prepared using this description after collection of plant samples i have to measure 200 milligram and grind it with liquid nitrogen then transfer all ground plant samples into an append of tube and add 700 microliters of sit up lysis buffer vortex the mixture then incubate at 65 degrees Celsius for 20 minutes in a water bath after incubation centrifuge samples at 10 000 rpm for 10 minutes after centrifuge transfer supernatant to a clean tube and add an equal volume of chloroform and isoamyl alcohol after addition of chloroform and iso-mine alcohol mix it well using vortex and centrifuge at 10 000 rpm for 10 minutes after centrifuge you will see an aqueous upper layer and an organic bottom layer now transfer the aqueous layer to a clean tube while transferring the quest layer...
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