How you can separate leaf pigments by a process known as chromatography? In this example I'm going to use spinach leaves so the first step is for you to obtain your plant that contains the pigments you wish to separate break up one of the leaves and de little pieces and place it in a mortar add a few drops of acetone and a little bit of sand as this helps with grinding up the leaves use the pestle mortar to grind up the leaves and to release the pigments if it helps here you can add a few more drops of acetone but ultimately the objective is to obtain a concentrated pigment in a liquid at the bottom of the mortar. Chromatography Paper after you've done this then you need to prepare your chromatography paper now this does actually work best with thin layer chromatography paper but you can also use regular chromatography paper to prepare you're going to obtain a pencil and it is important that it's a pencil not a pen because a pen contains other pigments use the pencil
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